141 Mouse anti-human Apolipoprotein E monoclonal antibody, clone 3D12 1 ml
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287 EUR
Monoclonal antibody (clone 3D12, IgG1 fraction) anti-human Apolipoprotein E (APOE)
Specificity
Human Apolipoprotein E (all alleles). Reacts with LDL and VLDL. No cross-reactivity to other human plasma proteins
Preparation
Ammonium sulphate precipitation. DEAE-cellulose chromatography with gradient elution
Format
Lyophilised from solution containing 0.5 mg antibody, 1% mannitol, 0.15 M sodium chloride, 0.01 M sodium phosphate
Applications
Western blotting, ELISA (x1000), Immunohistochemistry (x100), IHC 1:25 (after deparaffinization, rehydration and treatment with pronase E ) (1)
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
References
1) Klaver CCW, Kliffen M, van Duijn CM, Hofman A, Cruts M, Grobbee DE, van Broeckhoven C, de Jong PTVM (1998) Genetic Association of Apolipoprotein E with Age-Related Macular Degeneration. Am. J. Hum. Genet. 63:200-206