148 Collagen type IV (bovine, atelocollagen, sterile, lyophilized)
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Artikelnummer: 148
Bovine collagen type IV atelocollagen, sterile, 1 mg – basement-membrane collagen for coating
Type IV collagen purified from bovine placenta by pepsin solubilisation and salt fractionation (>90%), supplied sterile, lyophilised and salt-free. Collagen IV is the network-forming collagen of basement membranes, so coating with it gives epithelial, endothelial, muscle and neuronal cells a more physiological substrate than type I and improves attachment and differentiation of many primary cells and iPSC derivatives. The sterile 1 mg vial is ready for cell culture; dissolve in dilute acetic acid as described below.
Catalog Number 148
Amount 1 mg
Source
Bovine placenta
Purity
Bovine collagen type IV > 90%. bovine collagen type I and III < 10%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type IV was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating)
Reconstitution
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4°C
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
