148 Collagen type IV (bovine, atelocollagen, sterile, lyophilized)
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Article Number: 148
Description
Collagen type IV protein purified from bovine placenta (atelocollagen). Sterile and lyophilized
Catalog Number 148
Amount 1 mg
Source
Bovine placenta
Purity
Bovine collagen type IV > 90%. bovine collagen type I and III < 10%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type IV was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating)
Reconstitution
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4°C
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
