789 Apolipoprotein A1 (human) 0.5 mg

Full length native Apolipoprotein A1 protein purified from human plasma

Source

Human plasma. Donors tested negative for HBsAg, HBc, HIV-I, HIV-II and HCV antibodies

Purity

At least 90% pure, judged by SDS-PAGE

Format

Lyophilized from 0.05 M Na-chloride, 0.01 M Na-carbonate, pH 10.0

Purification

Ultracentrifugation in density gradient. Delipidation with diethyl ether and ethanol. Gel filtration on Sephacryl S-300

Applications

Apolipoprotein A1 standard. Antigen for immunisation (1) and antibody production. Coating material for cell culture studies. ELISA

Reconstitution

Sterile pure water or PBS

Storage

Storage of lyophilized product 2 years at -20°C. Storage of reconstituted product 1 month at +4°C, 1 year at -20°C or lower. Avoid multiple freeze-thaw cycles

Citations

1. Engelbertsen D, Rattik S, Knutsson A, Björkbacka H, Bengtsson E, Nilsson J. Induction of T helper 2 responses against human apolipoprotein B100 does not affect atherosclerosis in ApoE-/- mice. Cardiovasc Res. 2014 Jul 15;103(2):304-12. doi: 10.1093/cvr/cvu131. Epub 2014 May 27. PubMed PMID: 24866382

2. Negi P, Lövgren J, Malmi P, Sirkka N, Metso J, Huovinen T, Brockmann EC, Pettersson K, Jauhiainen M, Lamminmäki U. (2015) Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library. Clin Biochem. 2016 Apr;49(6):472-479. doi: 10.1016/j.clinbiochem.2015.11.020. Epub 2015 Dec 2 PMID: 26656638