393 Mouse anti-human collagen type I monoclonal antibody, clone DBH 428 1 ml
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492 EUR
Mouse anti-human collagen type I monoclonal antibody, clone DBH 428, 1 ml
Mouse monoclonal antibody that binds native human collagen type I with no reactivity to collagens II, III, IV or V. Purified by ammonium-sulphate precipitation and anion-exchange chromatography, lyophilised, 1 ml after reconstitution. For ELISA, immunohistochemistry on frozen sections and staining of collagen I deposited in cell culture; the polyclonal alternative is 762 goat anti-human collagen type I.
Specificity
Binds Human Collagen type I. No affinity to human collagens of type II, III, IV and V
Preparation
Ammonium sulfate precipitation and purification by anion-exchange chromatography
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
ELISA (x1000), Immunohistochemistry (frozen section) (x20), Cell culture. Not suitable for denaturing conditions
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower. Storage of reconstituted antibodies 10 days at +4°C
