182 Mouse anti-human Apolipoprotein B monoclonal antibody, clone 4C11 0.5 mg
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492 EUR
Mouse anti-human apolipoprotein B-100 monoclonal antibody, clone 4C11 (T3 domain), 0.5 mg
Mouse IgG1 monoclonal antibody directed to the central T3 thrombolytic fragment of human apolipoprotein B-100, a region absent from ApoB-48, with no reactivity to other plasma proteins. Purified IgG, lyophilised, 0.5 mg. For ELISA, immunohistology and capture of ApoB-100-containing lipoproteins; because it binds a different domain from the N-terminal clones 12G10 (447) and 5F8 (387) it is a natural partner for sandwich assays.
Specificity
Specific against T3 fragment, the middle thrombolytic peptide of human Apolipoprotein B (LDL). No reactivity to other human plasma proteins
Preparation
Precipitation by sodium sulphate and anion-exchange chromatography (DEAE-cellulose) with gradient elution
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
Detection of Apolipoprotein B (LDL) in human plasma or serum. Extraction of Apo-B containing lipoproteins from human plasma. ELISA (x1000), Immunohistology (x100)
Reconstitution
Use 1 ml sterile pure water, aliquot and store at -20°C or lower
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
