214 Rabbit anti-human collagen type II polyclonal antibody 0.5 ml
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492 EUR
Rabbit anti-human collagen type II (cartilage collagen, COL2A1) polyclonal antibody, cross-adsorbed, 0.5 ml
Rabbit polyclonal raised against native human articular-cartilage collagen type II and made type-specific by affinity purification and cross-adsorption on human collagens I, III, IV and V (<5% cross-reactivity) and plasma proteins. For immunohistochemistry on frozen sections (~1:20) and ELISA (~1:3000) in cartilage and osteoarthritis research, chondrogenic differentiation of stem cells, cartilage tissue engineering and characterisation of type II collagen preparations. Lyophilised; reconstitute with 0.5 ml water.
Specificity
Binds human collagen type II. Cross-reactivity to other human collagen type I, III, IV and V <5%. No cross-reactivity to human plasma proteins
Preparation
Affinity purification on immobilized collagen type II with cross-absorption to immobilised human blood plasma proteins and human collagen type I, III, IV and V
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
ELISA (x3000), Histochemistry (frozen sections) (x20). Not suitable for denaturing and dissociating conditions
Reconstitution
Use 0.5 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies for 2 years at -20°C or lower
