879 Mouse anti-human CD61 (beta-integrin, Platelet GPIIIa, VNR beta-chain 3) monoclonal antibody, clone CRC54 1 ml
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Mouse anti-human CD61 (integrin β3 / platelet GPIIIa) monoclonal antibody, clone CRC54, 1 ml
Mouse IgG1κ monoclonal against CD61, the β3 integrin subunit shared by the platelet fibrinogen receptor GPIIb/IIIa (αIIbβ3) and the vitronectin receptor αVβ3. CRC54 binds an N-terminal epitope of GPIIIa and reacts preferentially with the ligand-occupied, activated conformation (a LIBS-type epitope), which makes it useful not only for identifying platelets, megakaryocytes and megakaryoblasts by flow cytometry, immunofluorescence and IHC on frozen sections, but also for detecting platelet activation and for studying Glanzmann thrombasthenia (no reaction with GPIIIa-deficient platelets). Purified, lyophilised; reconstitute with 1 ml water.
Specificity
Reacts with platelet glycoprotein gpIIIa/VNR beta chain. This glycoprotein is the common beta chain (beta3 Integrin) of the cytoadhesins, gpIIbIIIa and VNR. Reacts with platelets and megakaryocytes. The antibody reacts preferentially with the activated/occupied form of platelet GPIIb-IIIa (ligand-induced binding site or LIBS epitope) and dissociated form of GPIIIa. CRC54-epitope is located within the N-terminal region of GPIIIa (first 100 amino acids). CRC54 does not react with platelets of patients with Glanzmann thrombastenia
Preparation
Ammonium sulphate precipitation and DEAE-cellulose chromatography with gradient elution
Format
Purified and lyophilized. Contains azide and PBS. The azide concentration in reconstituted solution is 3 mM
Applications and recommended dilutions
Immunoassay, Immunofluorescence, FACS, IHC (frozen)
Antibody can be used for:
-Determination of thrombocytes in blood.
-Identification of megakaryocytes and -blasts in tissue.
-Immunoblotting under reduced and nonreduced conditions.
-Detection of conformational changes of GPIIb-IIIa on activated platelets
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
