656 Mouse anti-human CD42b (Platelet GPIb) monoclonal antibody, clone VM16d 1 ml

Monoclonal antibody anti-human CD42b (Platelet GPIb) (clone VM16d, IgG1 kappa fraction)

Specificity

The monoclonal antibody is directed against the CD42b antigen, platelet glycoprotein GPIb, that serves as a receptor for von Willebrand factor and as a high affinity thrombin receptor. The antigen is expressed on human platelets and megakaryocytes. The antigen is absent or present in very low levels on platelets of patients with the Bernard-Soulier syndrome. VM16d inhibits low conc. thrombin (<0.1 U/ml) induced platelet aggregation and decreases thrombin binding to platelets. F(ab')2 and F(ab') fragments are equally effective as whole intact antibodies. the VM16d-epitope is located within the 45 kDa N-terminal domain of GPIb alpha- chain. VM16d does not effect platelet interaction with von Willebrand factor, leading to the conclusion that the antibody reacts with or nearby the thrombin receptor site

Preparation

Ammonium sulphate precipitation and DEAE-cellulose chromatography with gradient elution

Format

Purified and lyophilized. Contains azide and PBS. The azide concentration in reconstituted solution is 3 mM

Applications

Analysis of platelet aggregation and detection of GPIb expression. The antibody is also useful for functional studies

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower

Citation

Qi Y et al. Novel antibodies against GPIb alpha inhibit pulmonary metastasis by affecting vWF-GPIb alpha interaction. Corpus ID: 108285710