387 Mouse anti-human Apolipoprotein B monoclonal antibody, clone 5F8 1 ml
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492 EUR
Mouse anti-human apolipoprotein B (ApoB-100 / ApoB-48) monoclonal antibody, clone 5F8, 1 ml
Mouse monoclonal antibody directed to the N-terminal T4 fragment of human apolipoprotein B, an epitope shared by ApoB-100 of LDL/VLDL and the intestinal ApoB-48 of chylomicrons, with no reactivity to other plasma proteins. Purified IgG, lyophilised, 1 ml after reconstitution. For ELISA, immunohistology and immunocapture of ApoB-containing lipoproteins; clone 12G10 (447) and the polyclonal 843 recognise the same protein and can be paired in sandwich assays.
Specificity
Specific against T4 fragment, the N-terminal thrombolytic peptide of human Apolipoprotein B (LDL) (B100 and B48). No reactivity to other human plasma proteins
Preparation
Precipitation by sodium sulphate and anion-exchange chromatography (DEAE-cellulose) with gradient elution
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
Detection of Apolipoprotein B (LDL) in human plasma or serum
Extraction of Apo-B containing lipoproteins from human plasma
ELISA (x1000), Immunohistology (x100)
Reconstitution
Use 1 ml sterile pure water, aliquot and store at -20°C or lower
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
