223 Collagen type I and III (porcine) 10 mg

Porcine collagen type I and III purified from porcine tendons

Source

Porcine tendons. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation

Purity

Porcine collagens: type I and III 95%, other collagen types below 1%. Non-collagen proteins below 0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Standard for immunological assays. Coating material for cell culture studies. May not be suitable for 3-D gel formation

Pig cell migration assay (1)

Reconstitution

Use acetic acid, pH 2.5 in concentration from 0.02 to 0.5M. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein at -20°C. Storage of reconstituted protein 1 month at +4°C

Citations

1. O'Brien K, Bhatia A, Tsen F, Chen M, Wong AK, et al. (2014) Identification of the Critical Therapeutic Entity in Secreted Hsp90a That Promotes Wound Healing in Newly ReStandardized Healthy and Diabetic Pig Models. PLoS ONE 9(12): e113956. doi:10.1371/ journal.pone.0113956 PMID: 25464502

2. Yang Zhang, Yi Chen, Bo Zhao, Jianping Gao, Leilei Xia, Fangyu Xing, Yingjun Kong, Yongchao Li and Guifeng Zhang. Detection of Type I and III collagen in porcine acellular matrix using HPLCﾖMS. Regenerative Biomaterials, 2020, 1-6