601 Collagen type I (goat) 10 mg
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Goat collagen type I (caprine atelocollagen), 10 mg
Pepsin-solubilised type I collagen purified from goat tissue and lyophilised salt-free. Caprine collagen is a less common species variant that is useful as a species-specific standard and antigen (for example when raising or validating anti-collagen antibodies against goat tissue), as a platelet-aggregation agonist and as a coating substrate; it is not recommended for 3D gel formation. Other species in the same series: bovine (cat. 631), porcine (cat. 223) and canine (cat. 739).
Source
Goat tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation
Purity
90% SDS-PAGE
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils
Storage
Shipping at ambient temperature. Storage 2 years at -20°C or lower
