224 Collagen type III (rat) 10 mg
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Rat collagen type III
Source
Newborn rats. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type III was purified by using differential salt precipitation
Purity
Rat collagen type III 60%. Other rat collagens 40%. Non-collagen proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Rat collagen (type III) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C