533 Mouse anti-human collagen type IV monoclonal antibody, clone MC4-HA 1 ml
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492 EUR
Mouse anti-human collagen type IV monoclonal antibody, clone MC4-HA (IgM), 1 ml
Mouse IgM monoclonal that recognises human collagen type IV, the network-forming collagen of basement membranes, both in its native form and after heat denaturation, with no cross-reaction to the fibrillar collagens I, II, III or V. Lyophilised (reconstitute with 1 ml water) for ELISA at ~1:1000 and for immunohistochemistry on acetone-fixed frozen sections at ~1:20, where it outlines basement membranes of vessels, epithelia, muscle fibres and tumour nests.
Specificity
Binds Human Collagen type IV in native and heat-denatured state. No cross-reactivity to human collagen I, II, III and V
Preparation
PEG-6000 precipitation and purification by gel chromatography
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
ELISA (x1000), Immunohistochemistry (x20). For immunohistochemistry on frozen sections fixation with acetone for 5 min at -20°C is recommended.
Reconstitution
Use 1 ml sterile pure water
Storage
Storage of lyophilized antibodies 2 years -20°C or lower
