153 Goat anti-human collagen type III polyclonal antibody 1 ml
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492 EUR
Goat anti-human collagen type III (COL3A1) polyclonal antibody, affinity-purified, 1 ml
Type-specific goat antibody against native human collagen type III, affinity-purified on immobilised collagen III and cross-absorbed against collagens I, II, IV and V and human plasma proteins. Lyophilised, 1 ml after reconstitution. Suited to ELISA and immunostaining of frozen sections and extracellular matrix in cell culture. Antigen available as 785 human collagen type III.
Specificity
Binds human collagen type III. Cross-reactivity to human collagen type I, II, IV and V <5%. No cross-reactivity to human plasma proteins
Preparation
Affinity purification on immobilized human collagen type III with cross-absorption using immobilised human plasma proteins and human collagen type I, II, IV and V. Overall process is designed to obtain a high purity type-specific antibody to native 3D collagen.
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
Detection of human collagen type III by ELISA (x3000), Histochemistry (frozen sections) (x20). Immunostaining of ECM in cell culture. Not suitable for denaturing and dissociating conditions
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibody 2 years at -20°C or lower
