153 Goat anti-human collagen type III polyclonal antibody 1 ml

Polyclonal goat antibody anti-human collagen type III (COL3A1)

Specificity

Binds human collagen type III. Cross-reactivity to human collagen type I, II, IV and V <5%. No cross-reactivity to human plasma proteins

Preparation

Affinity purification on immobilized human collagen type III with cross-absorption using immobilised human plasma proteins and human collagen type I, II, IV and IV. Overall process is designed to obtain a high purity type-specific antibody to native 3D collagen.

Format

Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8

Applications and recommended dilutions

Detection of human collagen type III by ELISA (x3000), Histochemistry (frozen sections) (x20). Immunostaining of ECM in cell culture. Not suitable for denaturing and dissociating conditions

Reconstitution

Use 1 ml sterile pure water

Storage

Shipping at ambient temperature. Storage of lyophilized antibody 2 years at -20°C or lower