655 Streptavidin 5 mg

Tetrameric streptavidin (53 kDa) purified from culture of Streptomyces avidinii by ion-exchange chromatography on CM-cellulose and affinity chromatography on iminobiotin-Sepharose. Non-glycosylated and with a near-neutral isoelectric point, streptavidin gives lower non-specific background than egg-white avidin while keeping the same femtomolar affinity for biotin. Supplied lyophilised, 5 mg per vial, for coating ELISA microplates and beads, preparing immunosorbents and capturing or detecting biotinylated antibodies, proteins and nucleic acids in ELISA, dot blot, Western blotting and immunohistochemistry.

Catalog Number 655

Amount 5 mg

Cell culture of a Streptomyces avidinii strain

Ion-exchange chromatography on CM-cellulose followed by affinity chromatography on iminobiotin-Sepharose

Single band in reducing SDS-PAGE. Binding to immobilised biotin >95%. Activity 17 units/mg protein (1 unit binds 1 µg of D-biotin). Molecular weight 53 kDa; E (280 nm, 1%) = 3.0

Lyophilised from 1.0 ml of 0.01 M sodium phosphate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 7.5

Coating of microplates and solid phases. Ligand for immunosorbent preparation. Detection of biotinylated proteins in ELISA, dot blot, Western blotting and immunohistochemistry

Dissolve in distilled water or buffered saline. Reconstituted protein: 10 days at +4°C or 1 year at -20°C

Shipping at ambient temperature. Store lyophilised protein at -20°C or lower

RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE