270 Urokinase (uPA) colorimetric substrate
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Urokinase (uPA) chromogenic substrate pyroGlu-Gly-Arg-pNA·HCl, 100 mg
L-Pyroglutamyl-glycyl-L-arginine-p-nitroanilide hydrochloride (Mw 499), a chromogenic peptide substrate for urokinase-type plasminogen activator, 97 % pure by HPLC and lyophilised. Hydrolysis releases p-nitroaniline, read at 405 nm, giving a simple kinetic assay of uPA activity for enzyme quality control, inhibitor screening and plasminogen-activator research. Active enzyme available as 840 recombinant human urokinase.
| Catalog Number 270 | Amount 100 mg |
Purity
97% HPLC
Format
Lyophilized
Applications
Activity assay. Activity is measured by increase of optical density (405 nm) at 37°C at 0.025 min-1 in buffer (0.14M NaCl, 0.01M Na-phosphate pH 7.5, 0.2 mM of substrate and 100 IU/ml of urokinase (cat 282))
Reconstitution
Dissolve in distilled water.
Storage
Shipping at ambient temperature. Lyophilized product is stored for 2 years at -20°C, dry and dark. Reconstituted product is kept at +4°C for 2 months
