471 Polyclonal rabbit antibody anti-human collagen type VI 1 ml
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492 EUR
Rabbit anti-human collagen type VI polyclonal antibody, pre-adsorbed, 1 ml
Rabbit polyclonal antibody against human collagen type VI, the beaded-filament microfibrillar collagen of connective tissue, pre-adsorbed against human plasma proteins and collagens I, III, IV and V. IgG fraction (about 4 mg), lyophilised, 1 ml after reconstitution. Reacts with native and heat-denatured collagen VI; for ELISA and immunohistochemistry of muscle, skin and basement-membrane-associated matrix. The bovine antigen is listed as 231.
Specificity The antibody reacts with native and heat-denatured human collagen type VI. Reaction in competitive ELISA:
Human collagen type VI 100%
Human collagen type I, III 5%
Human collagen type IV and V 10%
Human plasma proteins do not interfere with binding of antibody to collagen
Preparation Pre-adsorption against human blood plasma proteins and human collagen types I, III, IV and V. Isolation of antibody fraction by sulphate precipitation and ion-exchange chromatography
Format Lyophilized rabbit IgG about 4 mg. Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions ELISA (x4000), Immunohistochemistry (x20)
Reconstitution Use 1 ml sterile pure water
Storage Shipping at ambient temperature. Storage of lyophilized antibodies at -20°C or lower
