219 Protein G agarose 5 ml

Recombinant Protein G immobilized on 4% agarose (slurry)

Format

Slurry containing 0.1 M NaCl, 0.1 M sodium borate, 0.01 M sodium azide, pH 8.0

Ligand

Recombinant Protein G

Matrix

4% cross-linked agarose

Specificity

Binds Fc-fragment of most subclasses of IgG of human, mouse, rat, rabbit, pig, goat and sheep. Does not bind human IgM, IgA, IgD or IgE. Albumin-binding domain deleted

Binding Capacity

Human IgG 10 mg per 1 ml gel

Applications

Affinity chromatography of mammalian immunoglobilins (whole molecules, Fc-fragments or immune complexes). Selective extraction of immunoglobulin IgG from human serum. Estimation of the IgG (antibody) content in immune sera and ascitic fluids

Recommended buffers

Binding buffer: 0.15 M sodium chloride, 0.05 M sodium phosphate, pH 7.0

Elution buffer: 0.1 M sodium chloride, 0.1 M sodium acetate, pH 2.5

Storage

Shipping at ambient temperature. Storage at +4°C for 1 year. Do not freeze